Onkologische Forschung

    Core Unit Metabolomics


    The aim of the core unit Cancer Metabolomics is to analyse changes in cellular metabolism in cancer cells. The unit is developing suitable technologies to accurately determine metabolite concentration in cancer cell lines and tumour samples by mass spectrometry (LC/MS, Thermo Fisher Q-Exactive Orbitrap). We are also establishing protocols for the labelling of cancer cells with stable isotope tracers (i.e. glucose or glutamine) for isotopomer spectral analysis (ISA) and metabolic flux analysis (MFA). These techniques allow the identification of metabolic intermediates derived from different nutrients and can reveal altered metabolic flux in cancer cells. 

    Recent Publications

    Bohnert S, Reinert C, Trella S, Schmitz W, Ondruschka B, Bohnert M.
    Metabolomics in postmortem cerebrospinal fluid diagnostics: a state-of-the-art method to interpret central nervous system-related pathological processes.
    Int J Legal Med. 2021 Jan;135(1):183-191.

    Sridhar S, Schmitz W, Hiltunen JK, Venkatesan R, Bergmann U, Kiema TR, Wierenga RK.
    Crystallographic binding studies of rat peroxisomal multifunctional enzyme type 1 with 3-ketodecanoyl-CoA: capturing active and inactive states of its hydratase and dehydrogenase catalytic sites.
    Acta Crystallogr D Struct Biol. 2020 Dec 1;76(Pt 12):1256-1269.

    Jessen C, Kreß JKC, Baluapuri A, Hufnagel A, Schmitz W, Kneitz S, Roth S, Marquardt A, Appenzeller S, Ade CP, Glutsch V, Wobser M, Friedmann-Angeli JP, Mosteo L, Goding CR, Schilling B, Geissinger E, Wolf E, Meierjohann S.
    The transcription factor NRF2 enhances melanoma malignancy by blocking differentiation and inducing COX2 expression.
    Oncogene. 2020 Sep 25. doi: 10.1038/s41388-020-01477-8. Online ahead of print. PMID: 32978520 

    Oelschlaegel D, Weiss Sadan T, Salpeter S, Krug S, Blum G, Schmitz W, Schulze A, Michl P.
    Cathepsin Inhibition Modulates Metabolism and Polarization of Tumor-Associated Macrophages.
    Cancers 2020 Sep 10;12(9):E2579. 

    Rajeeve K, Vollmuth N, Janaki-Raman S, Wulff TF, Baluapuri A, Dejure FR, Huber C, Fink J, Schmalhofer M, Schmitz W, Sivadasan R, Eilers M, Wolf E, Eisenreich W, Schulze A, Seibel J, Rudel T.
    Reprogramming of host glutamine metabolism during Chlamydia trachomatis infection and its key role in peptidoglycan synthesis.
    Nat Microbiol. 2020 Nov;5(11):1390-1402. 

    Sah-Teli SK, Hynönen MJ, Sulu R, Dalwani S, Schmitz W, Wierenga RK, Venkatesan R.
    Insights into the stability and substrate specificity of the E. coli aerobic β-oxidation trifunctional enzyme complex.
    J Struct Biol. 2020 Jun 1;210(3):107494. 

    Kaymak I, Maier CR, Schmitz W, Campbell AD, Dankworth B, Ade CP, Walz S, Paauwe M, Kalogirou C, Marouf H, Rosenfeldt MT, Gay DM, McGregor GH, Sansom OJ, Schulze A.
    Mevalonate pathway provides ubiquinone to maintain pyrimidine synthesis and survival in p53-deficient cancer cells exposed to metabolic stress.
    Cancer Res. 2020 Jan 15;80(2):189-203.