Onkologische Forschung

    Core Unit Metabolomics


    The aim of the core unit Cancer Metabolomics is to analyse changes in cellular metabolism in cancer cells. The unit is developing suitable technologies to accurately determine metabolite concentration in cancer cell lines and tumour samples by mass spectrometry (LC/MS, Thermo Fisher Q-Exactive Orbitrap). We are also establishing protocols for the labelling of cancer cells with stable isotope tracers (i.e. glucose or glutamine) for isotopomer spectral analysis (ISA) and metabolic flux analysis (MFA). These techniques allow the identification of metabolic intermediates derived from different nutrients and can reveal altered metabolic flux in cancer cells. 

    Recent Publications

    Kaymak I, Maier CR, Schmitz W, Campbell AD, Dankworth B, Ade CP, Walz S, Paauwe M, Kalogirou C, Marouf H, Rosenfeldt MT, Gay DM, McGregor GH, Sansom OJ, Schulze A.
    Mevalonate pathway provides ubiquinone to maintain pyrimidine synthesis and survival in p53-deficient cancer cells exposed to metabolic stress.
    Cancer Res. 2020 Jan 15;80(2):189-203. doi: 10.1158/0008-5472.CAN-19-0650. Epub 2019 Nov 19.
    Schmidt S, Gay D, Uthe FW, Denk S, Paauwe M, Matthes N, Diefenbacher ME, Bryson S, Warrander FC, Erhard F, Ade CP, Baluapuri A, Walz S, Jackstadt R, Ford C, Vlachogiannis G, Valeri N, Otto C, Schülein-Völk C, Maurus K, Schmitz W, Knight JRP, Wolf E, Strathdee D, Schulze A, Germer CT, Rosenwald A, Sansom OJ, Eilers M, Wiegering A.
    A MYC-GCN2-eIF2α negative feedback loop limits protein synthesis to prevent MYC-dependent apoptosis in colorectal cancer.
    Nat Cell Biol. 2019 Nov;21(11):1413-1424. doi: 10.1038/s41556-019-0408-0. Epub 2019 Nov 4.
    Doll S, Freitas FP, Shah R, Aldrovandi M, da Silva MC, Ingold I, Grocin AG, Xavier da Silva TN, Panzilius E, Scheel C, Mourão A, Buday K, Sato M, Wanninger J, Vignane T, Mohana V, Rehberg M, Flatley A, Schepers A, Kurz A, White D, Sauer M, Sattler M, Tate EW, Schmitz W, Schulze A, O'Donnel V, Proneth B, Popowicz GM, Pratt D, Angeli JPF, Conrad M.
    FSP1 is a glutathione-independent ferroptosis suppressor.
    Nature. 2019 Nov;575(7784):693-698. doi: 10.1038/s41586-019-1707-0. Epub 2019 Oct 21.
    Mäkelä AM, Hohtola E, Miinalainen IJ, Autio JA, Schmitz W, Niemi KJ, Hiltunen JK, Autio KJ.
    Mitochondrial 2,4-dienoyl-CoA reductase (Decr) deficiency and impairment of thermogenesis in mouse brown adipose tissue.
    Sci Rep. 2019 Aug 19;9(1):12038. doi: 10.1038/s41598-019-48562-x.
    Mayer AE, Löffler MC, Loza Valdés AE, Schmitz W, El-Merahbi R, Viera JT, Erk M, Zhang T, Braun U, Heikenwalder M, Leitges M, Schulze A, Sumara G.
    The kinase PKD3 provides negative feedback on cholesterol and triglyceride synthesis by suppressing insulin signaling.
    Sci Signal. 2019 Aug 6;12(593). pii: eaav9150. doi: 10.1126/scisignal.aav9150.