piwik-script

Deutsch Intern
    Core Unit Systems Medicine

    Equipment

    NGS Systems

    • NextSeq 500 (Illumina)
      • 130 to 400 million reads / sequencing run
      • Single-end- or paired-end sequencing
      • Read length up to 2x 150 bp
      • Maximal Output: 120 Gb
    • NextSeq 2000 (Illumina)
      • 400 to 1200 million reads / sequencing run
      • Single-end- or paired-end sequencing
      • Read length up to 2x 150 bp
      • Maximal Output: 360 Gb
    • MiniSeq (Illumina)
      • 8 bis 25 million reads / sequencing
      • Single-end- or paired-end sequencing
      • Read length up to 2x 150 bp
      • Maximal output: 7.5 Gb
    • MinION  (Oxford Nanopore)
      • 2.5 – 6 million reads / sequencing run
      • Read length: on average 5 kb (max. 2 Mb according to manufacturer's instructions)
      • Maximal output: 10 – 30 Gb

    Quantification & Quality Control of DNA/RNA

    • Nanodrop ND-2000 (ThermoFisher)

    • Nanodrop One (ThermoFisher)

    • Qubit 3.0 (ThermoFisher)

    • Bioanalyzer 2100 (Agilent)

    PCR Amplification

    • Gene Amp PCR 9700 (ABI / ThermoFisher)

    • CFX96 Real-Time System (Biorad)

    Single Cell Isolation

    • Chromium Controller for single cell partitioning via droplet microfluidics (10x Genomics)
      • 500 – 80.000 cells < 9 min
      • Capture rate for cells loaded: 65%
      • Doublet rate: 0.9% per 1000 cells
    • FACS Aria III (Becton Dickinson, BD)
      • ca. 1 x 96 well plate / 1 min
      • lasers: 405 nm, 488 nm, 561 nm, 633 nm
      • nozzles: 70 nm, 85 nm, 100 nm