Equipment
NGS Systems
- NextSeq 500 (Illumina)
- 130 to 400 million reads / sequencing run
- Single-end- or paired-end sequencing
- Read length up to 2x 150 bp
- Maximal Output: 120 Gb
- NextSeq 2000 (Illumina)
- 400 to 1200 million reads / sequencing run
- Single-end- or paired-end sequencing
- Read length up to 2x 150 bp
- Maximal Output: 360 Gb
- MiniSeq (Illumina)
- 8 bis 25 million reads / sequencing
- Single-end- or paired-end sequencing
- Read length up to 2x 150 bp
- Maximal output: 7.5 Gb
- MinION (Oxford Nanopore)
- 2.5 – 6 million reads / sequencing run
- Read length: on average 5 kb (max. 2 Mb according to manufacturer's instructions)
- Maximal output: 10 – 30 Gb
Quantification & Quality Control of DNA/RNA
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Nanodrop ND-2000 (ThermoFisher)
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Nanodrop One (ThermoFisher)
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Qubit 3.0 (ThermoFisher)
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Bioanalyzer 2100 (Agilent)
Single Cell Isolation
- Chromium Controller for single cell partitioning via droplet microfluidics (10x Genomics)
- 500 – 80.000 cells < 9 min
- Capture rate for cells loaded: 65%
- Doublet rate: 0.9% per 1000 cells
- FACS Aria III (Becton Dickinson, BD)
- ca. 1 x 96 well plate / 1 min
- lasers: 405 nm, 488 nm, 561 nm, 633 nm
- nozzles: 70 nm, 85 nm, 100 nm