Deutsch Intern
    Core Unit FACS

    Help & FAQ

    How should I send my samples?

    • Always on dry ice!

    How much RNA will I need?

    • Total RNA for rRNA-Depletion: ~ 1 µg
    • Total RNA for mRNA enrichment with Oligo-dT-capture beads: ~ 100 ng (down to 10 ng for high quality RNA)
    • Total RNA for ultra low input samples: ~ 10 pg - 10 ng (see also SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing)

    For which organisms do you have rRNA depletion solutions?

    • Illumina TruSeq Stranded Total RNA Gold:  human, mouse, rat
    • Illumina TruSeq Stranded Total RNA with Ribo-Zero Globin (Globin mRNA incl. rRNA Depletion):  human, mouse, rat
    • siTOOLs Biotech Pan-Prokaryote riboPOOL:  Bacteria / Archaea

    Tips for working with RNA:

    • Wear "fresh" disposable gloves
    • Clean bench and instruments (pipettes, ...) with cleaning solutions, such as RNaseZAP, RNase Away, ...
    • Spatial separation of the workplace for RNA work from workplaces working with RNase
    • Use sterile filter pipette tips
    • Use disposable sterile plastic as well as DEPC-treated reagents and glassware
    • follow protocols with short incubation times on ice and keep tubes closed whenever possible
    • if possible use of nuclease-free water (DEPC-treated or DNase / RNase-free)
    • Storage of RNA at -80 ° C in nuclease-free water (up to 1 year)
    • Link:  “The Do's and Don'ts of Working with RNA”